Using an electrochemical assay to determine the biofilm elasticity change as a response to toxicant exposure

International Conference on Frontier of Science and Society: Maritime Perspectives to Public Discourse

Daryono, Retno Novvitasari Hery^1 ; Feurtet-Mazel, Agnes^2 ; Bouletreau, Stephanie^3 ; Garabetian, Frederic^4

State Islamic University of Maulana Malik Ibrahim Malang, JawaTimur

65144, Indonesia^1

CNRS, EPOC, UMR 5805, Station Marine d'Arcachon, Arcachon

33120, France^2

Univ. Paul Sabatier (Toulouse 3), EcoLab UMR 5145, Toulouse, France^3

University of Bordeaux, EPOC, UMR 5805, Station Marine d'Arcachon, Arcachon

33120, France^4

关键词: Bio-film thickness;    Cell clustering;    Electrochemical assays;    Mean values;    Phototrophic biofilm;    Rotating disc electrode;    toxic;    Toxicant exposure;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/156/1/012005/pdf
DOI  :  10.1088/1755-1315/156/1/012005

来源: IOP

【 摘 要 】

Elasticity is a trait of biofilm physiognomy which relates to cell clustering and can be measured by means of an electrochemical assay based on rotating disc electrode (RDE). This study aimed at testing the hypothesis according to which exposure of phototrophic biofilm to toxicant could reduce its elasticity. We compared biofilms developed for 21 days, in four sets of 6 replicated experimental units, in absence and presence of isoproturon at two concentrations of the inoculating suspension of biofilm, 103 and 104 diatom cell mL-1. Biofilm thickness and elasticity were measured based on RDE assay, bacterial and diatom density were measured by microscope-based numerations.Very thin biofilms (