The axenic treatments for Kappaphycus alvarezii (Rhodophyta) seedling in laboratory culture

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Adharini, R.I.^1 ; Setyawan, A.R.^1 ; Jayanti, A.D.^1 ; Suadi, S.^1 ; Suyono, E.A.^2

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora 1A, Bulaksumur, Sleman Yogyakarta, Indonesia^1

Department of Biotechnology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman Yogyakarta, Indonesia^2

关键词: Axenic culture;    Bacterial contamination;    Culture medium;    Immersion method;    Immersion time;    Kappaphycus alvarezii;    Randomized design;    Rhodophyta;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012010/pdf
DOI  :  10.1088/1755-1315/139/1/012010

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

Obtaining an algae axenic culture in the culture medium are challenging. This study aims to determine the effectiveness of the application of several methods to reduce bacterial contamination in culture medium. The study was conducted using a complete randomized design in 3 stages, stage 1 using iodine immersion method with 4 treatments, stage 2 using immersion of antibiotics mixture with 3 different concentrations, stage 3 using mixture of antibiotic and medium with 3 concentration. The results showed that in stage 1, the lowest bacteria density was in treatment 4 (15 % betadine with 60') (675.27 idv•mm-2), stage 2 showed that treatment 1 (50 mg•L-1) had the lowest bacteria density (265.62 idv•mm-2). Stage 3 showed that treatment 3 (10 mg•L-1) had the lowest density of bacteria (24.78 idv•mm-2). Based on the ANOVA test, stage 1 has no significant difference (> 0.05), in stage 2 there was a significant difference with treatment 1 was the best treatment, in stage 3 there was a significant difference and treatment 4 was the best result. Iodine 15 % with 60' immersion time; immersion with 50 mg•L-1antibiotic, and mixture of medium with 10 mg•L-1antibiotic gave the best results in reducing bacterial contamination.