The reducibility of heLa cell viability by Sargassum polycystum extracts

Asean-Fen International Fisheries Symposium - 2017

Firdaus, M.^1 ; Setijawati, D.^1 ; Islam, I.^1 ; Nursyam, H.^1 ; Kartikaningsih, H.^1 ; Yufidasari, H.S.^1 ; Prihanto, A.A.^1 ; Nurdiani, R.^1 ; Jaziri, A.A.^1

Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Malang

65145, Indonesia^1

关键词: Bioactive compounds;    Brown seaweed;    Cervical cancers;    Cytotoxic agents;    Ethyl acetates;    Propanoic acid;    Sargassum polycystum;    Target proteins;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012064/pdf
DOI  :  10.1088/1755-1315/137/1/012064

来源: IOP

【 摘 要 】

Cervical cancer is the second largest cause of death-related cancer in women. The efficacy of cancer drugs is still low. Bioactive of brown seaweed has been studied by in vitro and in vivo as anticancer. The aim of this study was to evaluate the cytotoxicity of Sargassum polycystum extracts on HeLa cell, to recognize bioactive on extract and estimate the interaction between the bioactive and target protein. S. polycystum was found from Talango Island waters and HeLa cell was obtained from Indonesian Science Institute. Sample was extracted by ethanol, ethyl acetate and hexane, concentrated and finally, extracts were assayed on HeLa cell. The viability of this cell was quantified on ELISA-Reader. The bioactive compounds of the extract were elucidated by GC-MS. The interaction between bioactive and target protein was evaluated by using in silico method. The result showed that the lowest viability of HeLa cell on n-hexane extracts treatment. The n-hexane extract of this seaweed contained benzenepropanoic acid. This compound reduced HeLa cell viability by reducing of thrombin concentration. In conclusion, the benzene propanoic acid of S. polycystum was the cytotoxic agent and it is potential agent for anti-cervical cancer.