Detection of meca gene from methicillin resistant staphylococcus aureus isolates of north sumatera

3rd International Conference on Biological Sciences and Biotechnology

Nasution, Gabriella Septiani^1,2 ; Suryanto, Dwi^1 ; Kusumawati, R Lia^3,4

Department of Biology, University of Sumatera Utara, Kampus USU, Jln. Bioteknologi No. 1, Medan

20155, Indonesia^1

Medan Health Polytechnique, Ministry of Health, Jln. Jamin Ginting Km. 13.5, Lau Cih, Medan, Indonesia^2

Department of Microbiology, Faculty of Medicine, University of Sumatera Utara, Kampus USU, Jln. Universitas No. 1, Medan

20155, Indonesia^3

Clinical Research Unit, University of Sumatera Utara Hospital, Kampus USU, Jln. Dr. T. Mansyur No. 66, Medan

20154, Indonesia^4

关键词: Antibiotic resistance;    Beta-lactams;    Clinical microbiologies;    Freeze-thaw cycling;    Hospital acquired infection;    Methicillin-resistant staphylococcus aureus;    Multidrug resistants;    Nosocomial infection;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012026/pdf
DOI  :  10.1088/1755-1315/130/1/012026

来源: IOP

【 摘 要 】

Methicillin Resistant Staphylococcus aureus (MRSA) is a major pathogen associated with hospital-acquired infections (nosocomial infections). MRSA is a type of S. aureus resistant to the sub-group of beta-lactam antibiotics such as penicillin, cephalosporin, monobactam, and carbapenem. MRSA is resistant because of genetic changes caused by exposure to irrational antibiotic therapy. This study aimed to detect mecA gene in North Sumatra isolates of MRSA and to determine the pattern of antibiotic resistance in S.aureus isolates classified as MRSA by Vitek 2 Compact in the Central Public Hospital Haji Adam Malik, Medan. Samples were 40 isolates of S. aureus classified as MRSA obtained from clinical microbiology specimens. DNA isolation of the isolates was conducted by a method of freeze-thaw cycling. Amplification of mecA gene was done by PCR technique using specific primer for the gene. PCR products were visualized using mini-gel electrophoresis. The results showed that all MRSA isolates showed to have 533 bp band of mecA. Antibiotics test of Vitek 2 Compact showed that despite all isolates were resistant to beta-lactam antibiotics groups; the isolates showed multidrug resistant to other common antibiotics, such as aminoglycosides, macrolides, and fluoroquinolones. However, they were still sensitive to vancomycin (82.5% isolates), linezolid (97.5% isolates), and tigecycline (100% isolates).