Effects of hypoxia condition in embryogenic callus growth of soybean cell culture

International Conference on Agriculture, Environment, and Food Security

农业科学;生态环境科学

Damanik, R.I.^1 ; Manurung, B.H.^1 ; Bayu, E.S.^1

Agrotechnology Department, Faculty of Agriculture, Universitas Sumatera Utara, Medan

20155, Indonesia^1

关键词: Completely randomized designs;    Embryogenic callus;    Hypoxic condition;    Plant growth regulators;    T-tests;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/122/1/012056/pdf
DOI  :  10.1088/1755-1315/122/1/012056

来源: IOP

【 摘 要 】

The study was performed at Tissue Culture Laboratory, Agrotechnology Department, University of Sumatera Utara, to investigate the effect of plant growth regulator (PGR) and embryogenic callus performance soybean cultivars on hypoxia condition. This research had two stages, induction of embryogenic callus and analysis metabolism of callus after hypoxic condition with T-test. The analysis was used factorial Completely Randomized Design with two factors. The first factors were cultivars of soybean (Baluran, Gepak Kuning, and Grobogan) and the second factors were combinations of PGR (5 mg/l 2,4-D + 1 mg/l BAP, 10 mg/l 2,4-D + 1.5 mg/l BAP, and 15 mg/l 2,4-D + 2 mg/l BAP). The result showed the cultivars, combination of PGR, and interaction between cultivars and PGR gave significant effect to weight callus. The result of T-test showed that in hypoxic condition, POD enzyme exercise on Gepak Kuning's callus in 5 mg/l 2,4-D + 1 mg/l BAP was different before and after hypoxic condition.