Isolation of Rhizobium Bacteria from Forage Legumes for the Development of Ruminant Feed

International Ruminant Seminar: "Eco-friendly livestock production for sustainable agriculture"

农业科学;生态环境科学

Fuskhah, E.^1 ; Purbajanti, E.D.^1 ; Anwar, S.^1

Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang-Central-Java, Indonesia^1

关键词: Biochemical tests;    Electrical conductivity;    Forage legumes;    High salinity;    Leucaena leucocephala;    Ruminant feeds;    Soil salinity;    Total dissolved solids;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/119/1/012021/pdf
DOI  :  10.1088/1755-1315/119/1/012021

来源: IOP

【 摘 要 】

The aimed of the study was to explore the presence of Rhizobium bacteria along the northern coast of Central Java, to develop a saline-resistant legumes. Rhizobium bacteria is a mutualistic bacterium capable of symbiosis with legumes so that legumes crop yields increase. The research begins with sampling of soil and root nodule of forage legumes along the Northern Coast of Central Java including Tegal, Pekalongan, Semarang, Demak, Pati. Soil samples were analysed for salinity, Total Dissolved Solids, and pH. Rhizobium bacteria were isolated from the acquired root nodule, then identified by biochemical test to ensure that the isolates obtained were Rhizobium bacteria. The results showed that the five districts/municipal sites sampled by the soil have very low salinity to very high levels. The highest level of soil salinity was found in Demak (Sayung) which has an electrical conductivity value (EC) of 17.77 mmhos/cm. The EC values of legumes overgrown soils showed a low salinity level while bare soils have high salinity levels. Feed crops legumes that could be found in the northern coast of Central Java were Centrosema pubescens, Calopogonium mucunoides, Leucaena leucocephala, and Sesbania grandiflora. The study obtained 6 kinds of isolates of rhizobium bacteria isolated from forage legumes, included 1) Centrosema pubescens isolated from Pekalongan, 2) Centrosema pubescens isolated from Tegal, 3) Calopogonium mucunoides isolated from Pekalongan, 4) Leucaenaleucocephala isolated from Tegal, 5) Leucaena leucocephala isolated from Semarang, 6) Sesbania grandiflora isolated from Tegal.