Breeding of in-situ Petroleum Degrading Bacteria in Hangzhou Bay and evaluating for the In-situ repair effect

3rd International Conference on Advances in Energy Resources and Environment Engineering

能源学;生态环境科学

Lan, Ru^1,2 ; Lin, Hai^1 ; Qiao, Bing^2 ; Dong, Yingbo^1 ; Zhang, Wei^1 ; Chang, Wen^2

University of Science and Technology Beijing, Beijing

100083, China^1

China Waterborne Transport Research Institute, Beijing

100088, China^2

关键词: Degradation efficiency;    Degrading bacteria;    Degree of degradation;    Environmental conditions;    Environmental factors;    Marine accidents;    Nutrient elements;    Quantitative assessments;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/113/1/012115/pdf
DOI  :  10.1088/1755-1315/113/1/012115

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

In this paper, the restoration behaviour of the in-situ microorganisms in seawater and sediments to the marine accident oil spill was researched. The experimental study on the breeding of in-situ petroleum-degrading bacteria in the seawater and sediments of Hangzhou Bay and the restoration of oil spill were carried out. Making use of the reinforced microbial flora, combined with physical and chemical methods in field environment, petroleum degrading and restoration experiment were performed, the effect of the breeding of in-situ degrading bacteria was evaluated, and the standard process of in-situ bacteria sampling, laboratory screening, domestication and degradation efficiency testing were formed. This study laid a foundation for further evaluation of the advantages and disadvantages for the petroleum-degrading bacteria of Hangzhou Bay during the process of in-situ restoration. The results showed that in-situ microbes of Hangzhou Bay could reach the growth peak in 5 days with the suitable environmental factors and sufficient nutrient elements, and the degradation efficiency could reach 65.2% (or 74.8% after acclimation). And also the microbes could adapt to the local sea water and environmental conditions, with a certain degree of degradation. The research results could provide parameter support for causal judgment and quantitative assessment of oil spill damage.