Analysis on the Spatial Difference of Bacterial Community Structure in Micro-pressure Air-lift Loop Reactor

3rd International Conference on Advances in Energy Resources and Environment Engineering

能源学;生态环境科学

Wan, L.G.^1,2,3 ; Lin, Q.^2,3 ; Bian, D.J.^2,3 ; Ren, Q.K.^2,3 ; Xiao, Y.B.^2,3 ; Lu, W.X.^1

College of Environment and Resources, Jilin University, Changchun Jilin

130021, China^1

School of Water Conservancy and Environment Engineering, Changchun Institute of Technology, Changchun Jilin

130012, China^2

Jilin Provincial Key Laboratory of Municipal Wastewater Treatment, Changchun Institute, Technology University, Changchun Jilin, 130012, China^3

关键词: Airlift loop reactor;    Bacterial community structure;    Bacterial degradation;    Biodiversity indexes;    Denaturing gradient gel electrophoreses (DGGE);    Environmental conditions;    Shannon-wiener diversity indices;    Substrate concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/113/1/012053/pdf
DOI  :  10.1088/1755-1315/113/1/012053

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

In order to reveal the spatial difference of the bacterial community structure in the Micro-pressure Air-lift Loop Reactor, the activated sludge bacterial at five different representative sites in the reactor were studied by denaturing gradient gel electrophoresis (DGGE). The results of DGGE showed that the difference of environmental conditions (such as substrate concentration, dissolved oxygen and PH, etc.) resulted in different diversity and similarity of microbial flora in different spatial locations. The Shannon-Wiener diversity index of the total bacterial samples from five sludge samples varied from 0.92 to 1.28, the biodiversity index was the smallest at point 5, and the biodiversity index was the highest at point 2. The similarity of the flora between the point 2, 3 and 4 was 80% or more, respectively. The similarity of the flora between the point 5 and the other samples was below 70%, and the similarity of point 2 was only 59.2%. Due to the different contribution of different strains to the removal of pollutants, it can give full play to the synergistic effect of bacterial degradation of pollutants, and further improve the efficiency of sewage treatment.