Investigation of biological destruction of benzo[a]pyrene andpolycyclic aromatic hydrocarbons of biochar in soil

3rd International Conference Environment and Sustainable Development of Territories: Ecological Challenges of the 21st Century

生态环境科学;地球科学;经济学

Okunev, R.V.^1 ; Smirnova, E.V.^1 ; Sharipova, A.R.^1 ; Gilmutdinova, I.M.^1 ; Giniyatullin, K.G.^1

Institute of Environmental Sciences, Kazan Federal University, Kremlevskaya str., 18, Kazan

420008, Russia^1

关键词: Biological decomposition;    Contaminated soils;    Laboratory conditions;    Maximum permissible concentration;    Sensitive indicator;    Soil contamination;    Soil microbial activities;    Substrate-induced respirations;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/107/1/012121/pdf
DOI  :  10.1088/1755-1315/107/1/012121

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The biological decomposition of benzo[a]pyrene in the concentrations exceeding the MAC (maximum permissible concentration) level in soils by 2, 5 and 10 times was studied in laboratory conditions. The gray forest soil samples were contaminated with benzo[a]pyrene and incubated in optimum for bacterial growth soil moisture for 30 and 60 days. The residual amount of contaminant was monitored by HPLC after extraction with acetone-cyclohexane (2:1). Soil microbial activity was evaluated by measuring basal respiration (BR) and substrate-induced respiration (SID) rates of the soil by gas chromatography. The results of the experiment showed that in 60 days the amount of benzo[a]pyrene in contaminated soils decreased; however, this time was not enough for complete decomposition of pollutant. In this case, benzo[a]pyrene has a negative effect on the BR and SIR rates. Soil contamination affected the BR rate only at high doses (10 MPC), whereas the SIR was a more sensitive indicator of the toxic effect of the pollutant and significantly reacts already at concentrations at the level of 2 MPC. The combination of PAHs isolated from biochar has a strong negative effect on the values of BR and SIR.