Effect of medium and incubation time on production of AA, DHA and EPA from Aspergillus oryzae by solid state fermentation

2nd international Tropical Renewable Energy Conference 2017

Pinasthika, N.P.^1 ; Arbianti, R.^1 ; Utami, T.S.^1 ; Hermansyah, H.^1

Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok

16424, Indonesia^1

关键词: Aspergillus Oryzae;    Incubation time;    Malt extract agars;    Natural surfactants;    Optimal medium;    Potato dextrose agars;    Single cell oil;    Solid-state fermentation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/105/1/012104/pdf
DOI  :  10.1088/1755-1315/105/1/012104

来源: IOP

【 摘 要 】

Lately, artificial surfactant is more often used than natural surfactant for petroleum industry. The main advantages of using natural surfactant is because the continuous availability. Single Cell Oil, which utillizes one-cell microorganism, can be a solution, such as Aspergillus oryzae, to produce AA, DHA & EPA. A. oryzae was cultivated on Potato Dextrose Agar (PDA), Czapek Dox Agar (CDA) and Malt Extract Agar (MEA), and then the incubation times are 2,4,5,6 and 7 days in optimal medium. Lipids were extracted using ethanol and n-hexane. The characterization of lipid was done by gas chromatography (GC) method. The most optimal medium is CDA with a lipid concetration of 0,18 g. The best incubation time on CDA medium was 5 days with 0,216 g lipid content containing 0,123 g unsaturated fatty acid, 0,0613 g PUFA and 0,0615 g MUFA. The unsaturated fatty acid composition produced on the 5th day was 29.2% oleate; 29.1% linoleate and ± 0.046% EPA.