Rapid and reliable QuEChERS-based LC-MS/MS method for determination of acrylamide in potato chips and roasted coffee

59th International Meat Industry Conference

Stefanovi, S.^1 ; Dordevic, V.^1 ; Jelui, V.^2

Institute of Meat Hygiene and Technology, Kacanskog 13, Belgrade, Serbia^1

Veterinary Office of Bosnia and Herzegovina, Marala Tita 9a/II, Sarajevo, Bosnia and Herzegovina^2

关键词: Analytical method;    Fit for purpose;    Fitness for purpose;    Governmental agency;    Interlaboratory reproducibility;    Performance characteristics;    Quantitative determinations;    Regulatory control;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/85/1/012010/pdf
DOI  :  10.1088/1755-1315/85/1/012010

来源: IOP

【 摘 要 】

The aim of this paper is to verify the performance characteristics and fitness for purpose of rapid and simple QuEChERS-based LC-MS/MS method for determination of acrylamide in potato chips and coffee. LC-MS/MS is by far the most suitable analytical technique for acrylamide measurements given its inherent sensitivity and selectivity, as well as capability of analyzing underivatized molecule. Acrylamide in roasted coffee and potato chips wasextracted with water:acetonitrile mixture using NaCl and MgSO4. Cleanup was carried out with MgSO4and PSA. Obtained results were satisfactory. Recoveries were in the range of 85-112%, interlaboratory reproducibility (Cv) was 5.8-7.6% and linearity (R2) was in the range of 0.995-0.999. LoQ was 35 μg kg-1for coffee and 20 μg kg-1for potato chips. Performance characteristic of the method are compliant with criteria for analytical methods validation. Presented method for quantitative determination of acrylamide in roasted coffee and potato chips is fit for purposes of self-control in food industry as well as regulatory controls carried out by the governmental agencies.