Laccase Spontaneous Adsorption Immobilization: Experimental Studies and Mathematical Modeling at Enzymatic Fuel Cell Cathode Construction

2nd International Conference on Green Energy Technology

能源学;生态环境科学

Narkadeva, I.^1 ; Bogdanovskaya, V.A.^2 ; Vasilenko, V.A.^1 ; Fokina, E.A.^1 ; Koltsova, E.M.^1

Department of Informational Computing Technologies, D. Mendeleev University of Chemical Technology of Russia (MUCTR), Miusskaya sq., 9, Moscow

125047, Russia^1

Laboratory of Electrocatalysis and Fuel Cells, Russian Academy of Sciences A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Leninsky prospect, 31, Moscow

199071, Russia^2

关键词: Bioelectro-catalysis;    Diffusion equations;    Enzyme concentrations;    Experimental research;    Fractional derivatives;    Laccase immobilizations;    Second Fick's law;    Spontaneous adsorption;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/83/1/012017/pdf
DOI  :  10.1088/1755-1315/83/1/012017

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The activity of a bioeletrode is largely determined by the amount of enzyme adsorbed on its active layer, including the distribution of enzyme along thickness in the carrier layer. The distribution of enzyme is also required for calculations of the characteristics of bioelectrocatalysis process using a mathematical model. In the present article, on the basis of conducted experimental research a mathematical model of laccase immobilization by spontaneous adsorption on carbon-based sorbentsof different nature was developed. The model can be used to predict adsorption value and enzyme distribution in the layer of an adsorbent. The model includes the equations of the enzyme concentration changing due to its adsorption on the surface of the carbon material (CM) and the enzyme diffusion over the thickness of CM. The diffusion equation is based on the second Fick's law and contains fractional derivatives instead of the first oder derivative.