Comparison of two chemical extraction methods for proteins and polysaccharides of Spirogyra fluviatilis in extracellular polymeric substances

International Symposium on Resource Exploration and Environmental Science

地球科学;生态环境科学

Chang, Shuiping^1 ; Lee, Yichao^1

Department of Environmental Engineering, Kun Shan University, Tainan City, Taiwan^1

关键词: Chemical extraction method;    Extracellular polymeric substances;    Extraction method;    Extraction yield;    Large amounts;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/64/1/012122/pdf
DOI  :  10.1088/1755-1315/64/1/012122

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Under natural circumstances, Spirogyra fluviatilis excretes large amounts of extracellular polymeric substances (EPS) and forms a mucilage layer. This study used two algal EPS extraction methods such as NH4OH and formaldehyde-NaOH to compare the extraction yields of EPS and the levels of its primary components of polysaccharides and proteins. The results indicated that the extraction yields of EPS using formaldehyde-NaOH and NH4OH are 8.05 mg EPS/g-fresh algae and 5.26 mg EPS/g-fresh algae, respectively. The ratio of proteins to polysaccharides in EPS is around 0.24 to 0.34, which is not a significant difference between the two extraction methods. In other words, the level of polysaccharides is always higher than the level of proteins in EPS extraction with both two methods.