Adsorption of bovine serum albumin and urease by biochar

3rd International Conference on Energy Materials and Environment Engineering

能源学;生态环境科学

Wang, Wenjing^1 ; Chen, Lei^1 ; Zhang, Yipeng^1 ; Liu, Guocheng^1

Key Laboratory of Marine Environment and Ecology, Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao

266100, China^1

关键词: Adsorption amounts;    Bovine serum albumins;    High specific surface area;    Initial concentration;    Protein concentrations;    Soil enzymes;    Soil improvement;    Sorption rate;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/61/1/012143/pdf
DOI  :  10.1088/1755-1315/61/1/012143

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The application of biochar to soil improvement inevitably affects free soil enzymes. However, there is little information on the interaction of soil enzymes with biochar to our knowledge. We thus investigated the adsorption of bovine serum albumin (BSA) and urease onto two biochars from giant reed pyrolyzed at 300 and 600 °C (BCF300 and BCF600). The adsorption amount of BSA and urease on BCF300 and BCF600 was up to 45.6-209 mg/g and 75.3-808 mg/g, respectively, suggesting that the test proteins could be adsorbed onto the biochars effectively. The sorption rate of BSA and urease significantly decreased as the protein concentration increased, suggesting that their adsorption was nonlinear. For the same initial concentration (50 or 200 mg/L), the adsorption amount of BSA on the biochars was lower, only 25.9-60.5% of that of urease. The high specific surface area and hydrophobicity of the biochars may play important roles on the immobilization of the proteins by biochars. These findings will be helpful for better understanding the effects of biochar adding on the soil enzymes.