Detection of sulfonamide resistant bacteria and resistance genes in soils

3rd International Conference on Energy Materials and Environment Engineering

能源学;生态环境科学

Wu, Nan^1 ; Zhang, Weiyu^1 ; Liu, Huifen^2 ; Wang, Xiaobo^2 ; Yang, Fan^1 ; Zeng, Ming^3 ; Chen, Pinpin^1 ; Wang, Xiao^1

College of Engineering and Technology, Tianjin Agricultural University, Tianjin

300384, China^1

College of Agronomy and Resources and Environment, Tianjin Agricultural University, Tianjin

300384, China^2

College of Marine and Environment, Tianjin University of Science and Technology, Tianjin

300457, China^3

关键词: Agricultural soils;    Antibiotic resistance genes;    Antibiotic-resistant bacteria;    Environmental reservoirs;    Manure applications;    Positive correlations;    Soil organic matters;    Total viable counts;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/61/1/012075/pdf
DOI  :  10.1088/1755-1315/61/1/012075

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Manure application could accelerate the environmental dissemination of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in soils. In this study, the prevalence of sulfonamide resistant bacteria and resistance genes was investigated in agricultural soils to which organic manures had been applied in Tianjin, China. Anti-sulfonamide bacteria were found in the range of 3.29 × 104to 1.70 × 105CFU/g dry soil, occupying 1.5% to 2.2% of total viable counts. And sulI and sulII genes were detected in all sampling sites, with relative abundances of 5.69 × 10-5to 6.95 × 10-4and 4.28 × 10-4to 1.25 × 10-3respectively. No significant correlations between cultivable sulfonamide resistant bacteria and sul genes were found in this study. While sulI showed significant positive correlation with soil organic matter. Overall, the results highlight that soil plays an important role in resistance genes capture as the environmental reservoir.