Determination of total arsenic in fish by hydride-generation atomic absorption spectrometry: method validation, traceability and uncertainty evaluation

1st International Symposium on Green Technology for Value Chains 2016

工业技术;生态环境科学

Nugraha, W.C.^1 ; Elishian, C.^2 ; Ketrin, R.^2

Research Center for Clean Technology, Indonesian Institute of Sciences, Jalan Cisitu Sangkuriang Bandung

40135, Indonesia^1

Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan Puspiptek Serpong, Tangerang Selatan

15314, Indonesia^2

关键词: Arsenic contamination;    Arsenic determination;    Certified reference materials;    Hydride generation atomic absorption spectrometry;    International standards;    Microwave digestion system;    Sources of uncertainty;    Uncertainty evaluation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/60/1/012036/pdf
DOI  :  10.1088/1755-1315/60/1/012036

来源: IOP

【 摘 要 】

Fish containing arsenic compound is one of the important indicators of arsenic contamination in water monitoring. The high level of arsenic in fish is due to absorption through food chain and accumulated in their habitat. Hydride generation (HG) coupled with atomic absorption spectrometric (AAS) detection is one of the most popular techniques employed for arsenic determination in a variety of matrices including fish. This study aimed to develop a method for the determination of total arsenic in fish by HG-AAS. The method for sample preparation from American of Analytical Chemistry (AOAC) Method 999.10-2005 was adopted for acid digestion using microwave digestion system and AOAC Method 986.15 - 2005 for dry ashing. The method was developed and validated using Certified Reference Material DORM 3 Fish Protein for trace metals for ensuring the accuracy and the traceability of the results. The sources of uncertainty of the method were also evaluated. By using the method, it was found that the total arsenic concentration in the fish was 45.6 ± 1.22 mg.Kg-1with a coverage factor of equal to 2 at 95% of confidence level. Evaluation of uncertainty was highly influenced by the calibration curve. This result was also traceable to International Standard System through analysis of Certified Reference Material DORM 3 with 97.5% of recovery. In summary, it showed that method of preparation and HG-AAS technique for total arsenic determination in fish were valid and reliable.