Production of peptone from boso fish (Oxyeleotris marmorata) for bacterial growth medium

1st International Symposium on Green Technology for Value Chains 2016

工业技术;生态环境科学

Priatni, S.^1 ; Kosasih, W.^1 ; Budiwati, T.A.^1 ; Ratnaningrum, D.^1

Research Unit for Clean Technology, Indonesian Institute of Sciences Jalan Sangkuriang, Bandung

40135, Indonesia^1

关键词: Bacterial growth;    Fish peptones;    Growth of bacteria;    Nitrogen sources;    Optimum conditions;    Sodium dodecyl sulphate;    Soluble proteins;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/60/1/012009/pdf
DOI  :  10.1088/1755-1315/60/1/012009

来源: IOP

【 摘 要 】

Underutilized Oxyeleotris marmorata fish is abundant and widespread in Indonesia. The study aimed to use O. marmorata fish for peptone production using papain from dried latex of papaya fruit. The peptone was applied as nitrogen sources for bacterial growth. The resulted peptone was optimized at 50-65°C for 5-8 hr, using 0.1% of papain at pH 6.0. Characterization of peptone was based on the soluble protein content, N-amino content, % degree hydrolysis (DH), SDS PAGE profile and growth of bacteria Escherichia coli and Staphylococcus aureus. The results indicated that the optimum condition for hydrolysis was at 50°C for 7 hr (p