Characterization of thermostable chitinase from Bacillus licheniformis B2

2nd International Conference on Natural Resources and Life Sciences

生态环境科学

Jayanthi, N.^1 ; Purwanto, M.G.M.^1 ; Chrisnasari, R.^1 ; Pantjajani, T.^1 ; Wahjudi, A.^1 ; Sugiarto, M.^1

Department of Biology, Faculty of Biotechnology, University of Surabaya, Jl. Raya Kalirungkut, Surabaya

60292, Indonesia^1

关键词: Bacillus licheniformis;    Broad temperature ranges;    Chitin derivatives;    Chitinases;    Chitinolytic activity;    Degradation process;    Shellfish waste;    Thermophilic microorganisms;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/293/1/012030/pdf
DOI  :  10.1088/1755-1315/293/1/012030

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Chitinases is an enzyme capable of degrading chitin into oligomers to produce chitin derivatives products which are more useful. Thermostable-chitinase is of important in the relevant industrial application, since the degradation process oftently requires prety high temperature. This research report a characterization of chitinase isolated from thermophilic microorganism. The chitinase was obtained from Bacillus licheniformis B2 isolated from Ijen hot spring, East Java. It has the best chitinolytic activity at pH 7 when colloidal chitin was used as substrate. The enzyme exhibited activity in broad temperature range, from 50 °C to 70 °C, optimally at 55 °C. It was stable at 50 °C up to 90 min, at 60 °C up to 60 min and at 70 °C up to 30 min. At neutral pH this enzyme has negative charge but further purification is needed to determine its pI. The Km and Vmax of this chitinase for colloidal chitin were 101.96 mg mL -1 and 2.72 μmol (min mL)-1, respectively. Addition of NaCl, KNO3 and MgSO4 decreased the activity of chitinase following mixed inhibitor mode. This enzyme should be a good candidate for applications in the recycling of chitin waste.