The Applying of Yeast Saccharomyces Cerevisiae for Rapid Estimation of Surface-Active Substances' Toxicity

International science and technology conference "Earth science"

Vyatchina, O.F.^1 ; Zhdanova, G.O.^1 ; Xie, Beizhen^6 ; Ponomareva, A.L.^4^5 ; Stom, D.I.^1^2^3 ; Fialkow, V.A.^2 ; Kupchinsky, A.B.^2 ; Saksonov, M.N.^1

Irkutsk State University, Irkutsk, Russia^1

Baikal Mus. at the Irkutsk Sci. Center of the Siberian Branch of the Russian Academy of Sciences, Listvyanka, Russia^2

Irkutsk National Research Technical University, Irkutsk, Russia^3

Somov Institute of Epidemiology and Microbiology, 1 str. Selskaya, 690922, Russia^4

V.I. il'Ichev Pacific Oceanological Institute, 43 str. Baltiyskaya, Vladivostok

690041, Russia^5

Institute of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing

100191, China^6

关键词: Constant maintenance;    Conventional techniques;    Growth functions;    Rapid estimation;    Reproducibilities;    Surface active substances;    Test reactions;    Yeast Saccharomyces cerevisiae;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/272/3/032208/pdf
DOI  :  10.1088/1755-1315/272/3/032208

来源: IOP

【 摘 要 】

To assess the toxicity of several surface-active substances the foaming activity of the yeast Saccharomyces cerevisiae is proposed to use as biotest. The criterion of toxicity in this test was the suppression of foaming in the suspension of yeast with glucose. The proposed test reaction showed a higher sensitivity to surface-active substances compared with the survival rate and the growth function of yeast. The results of the toxicologic testing are collated with data obtained using conventional techniques. The benefits of a new test reaction are technical simplicity, rate, low material costs, lack of need for constant maintenance in the active state of the test-culture, good reproducibility.