Response Mechanism of Microbial Communities in River Sediments under Different Hydrodynamic Conditions

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Sui, Ming^1 ; Guan, Yonglin^1 ; Wei, Mingying^1 ; Zhu, Keyong^1 ; Li, Chunming^1

Sichuan Technology and Business College, Sichuan, Dujiangyan

611800, China^1

关键词: Acoustic doppler;    Flow field distribution;    High-throughput sequencing;    Hydrodynamic conditions;    Laboratory experiments;    Microbial communities;    PCR amplification;    Response mechanisms;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/5/052152/pdf
DOI  :  10.1088/1755-1315/252/5/052152

来源: IOP

【 摘 要 】

This paper studies the response of sedimentary microbial communities to different hydrodynamic conditions through laboratory experiments. The hydrodynamic conditions and flow field distribution in the experimental tank were measured and simulated by acoustic Doppler flowmeter and FLUENT software. The sediment DNA of different culture time and different flow velocity points was extracted and amplified by PCR amplification and high-throughput sequencing. The OTU sequence of the microbial organism.