The effects of lead on ATPase activity in liver of Pelophylax nigromaculata

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Huang, Minyi^1^2 ; Ma, Jinjin^2

College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Hunan, Loudi, China^1

Anhui Prov. Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anhui, Anqing, China^2

关键词: Aquatic animals;    ATP-ase activity;    Blood circulation;    Grinding fluids;    In-vivo experiments;    Lead concentration;    Pb concentration;    Treatment group;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/4/042065/pdf
DOI  :  10.1088/1755-1315/252/4/042065

来源: IOP

【 摘 要 】

Lead is a major heavy metal in sewage, which affects the growth, development, reproduction, endocrine and blood circulation of aquatic animals. The effects of lead on the activity of ATPase in liver of Pelophylax nigromaculata were studied. In vitro experiment, the grinding fluid of the liver of P. nigromaculata was mixed with the equal proportion of 0, 0.1, 10, 100, 1000 and 10000 ug/L lead. In vivo experiment, the liver was treated with 0, 10, 100 and 1000 g/L of lead for 30 d. The absorbance of the liver was measured by ATPase kit with enzyme labeling instrument and ATPase activity of the liver was calculated according to the formula. In vitro experiments, compared with the control group, the activity of ATP enzyme in lead treated group decreased significantly (p