The effect of purification on Snail (Achatina fulica) cellulase enzyme characteristic

International Conference on Sustainable Agriculture for Rural Development 2018

Dini, I.R.^1 ; Restuhadi, F.^2 ; Silaturahmi, K.^2

Department of Agrotechnology, Faculty of Agriculture, Universitas Riau, Kampus Bina Widya, KM 12, 5 Simpang Baru Panam, Pekanbaru, Indonesia^1

Department of Food Technology, Faculty of Agriculture, Universitas Riau, Kampus Bina Widya, KM 12, 5 Simpang Baru Panam, Pekanbaru, Indonesia^2

关键词: Ammonium sulphate;    Cellulase enzyme;    Completely randomized designs;    Energy source;    Optimum temperature;    Specific activity;    Specific substrates;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/250/1/012051/pdf
DOI  :  10.1088/1755-1315/250/1/012051

来源: IOP

【 摘 要 】

Snail is an organism that able to produce cellulase enzyme due to its ability to use cellulose for energy source. The purposes of this study were to obtain best concentration of ammonium sulphate in increasing cellulase enzyme activity, optimum temperature, and cellulase enzyme specific substrate from snail. The snail cellulase enzyme was concentrated using ammonium sulphate. The study was designed as Completely Randomized Design consisting seven treatments (30-90% of ammonium sulphate) and three replicates. The result showed that the activity of crude cellulase enzyme was 0,123 U/ml. The optimum cellulose enzyme activity was at 50% ammonium sulphate as 0,609 U/ml and the specific activity was 0,970 U/mg. Optimum temperature of the concentrated cellulose enzyme was at 50°C and the highest activity was in CMC substrate at 0,396 U/ml.