Test of the resistance of rhizobium bacteria to salinity for the development of food legume plants in coastal areas

International Conference on Sustainable Agriculture for Rural Development 2018

Fuskhah, E.^1 ; Purbajanti, E.D.^1 ; Anwar, S.^1

Faculty of Animal and Agricultural Sciences, Diponegoro University Semarang, Central Java, Indonesia^1

关键词: Bacteria resistance;    Biochemical tests;    Coastal area;    Environmental problems;    Legume plant;    Root nodules;    Saline media;    Soil salinity;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/250/1/012044/pdf
DOI  :  10.1088/1755-1315/250/1/012044

来源: IOP

【 摘 要 】

Soil salinity is one of serious environmental problems. Rhizobium is a mutualistic bacterium capable of symbiosis with legumes. The aim of the study was to test the resistance of rhizobium bacteria isolated from root nodules of food legumes against saline media. Root nodules were collected from coastal areas of the district of Tegal, Pemalang, Semarang, Pati, and Rembang in Central Java. Rhizobium bacteria were isolated from the acquired root nodules, and then their identity was confirmed by biochemical tests. Rhizobium bacteria resistance to salinity was determined by growing isolated colonies on a medium with different salt content.Results showed that food crop legumes found from coastal area were soybeans (Glycine max L. Merr), peanuts (Vigna sinensis L.), green beans (V. radiata L.), and long beans (V. unguiculata L.). There were6 types of Rhizobium bacterial isolated from the following food crop legumes: 1) soybean isolated from Pati, 2) soybean isolated from Rembang, 3) peanut isolated from Pemalang, 4) green bean isolated from Pemalang, 5) long bean isolated from Semarang,and 6) longbean isolated from Tegal. The salinity resistance was in the following order: rhizobium bacterial isolated from green beans, soybeans, peanuts, and long beans, respectively.