Utilization of "Narik Layang" medium as alternative in high school biology practicum

1st International Conference on Global Issue for Infrastructure, Environment & Socio-Economic Development

经济学;社会科学（总论）

Taharu, F.I.^1 ; Nuryadin, C.^2 ; Hanifa, L.^3 ; Arif, A.R.^4

Department of Biology Education, Universitas Muhammadiyah Buton, Jl. Muhammad Husni Thamrin, No. 30 Kota Bau-Bau, 93712, Indonesia^1

Department of Indonesian Language and Literature Education, Universitas Muhammadiyah Buton, Jl. Muhammad Husni Thamrin, No. 30 Kota Bau-Bau, 93712, Indonesia^2

Department of Accounting, Universitas Muhammadiyah Buton, Jl. Muhammad Husni Thamrin, No. 30 Kota Bau-Bau, 93712, Indonesia^3

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Indonesia^4

关键词: Alternative source;    Bacterial colonies;    Bacterial growth;    Beef extracts;    Control mediums;    Descriptive analysis;    Gram-staining;    Sample preparation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/235/1/012095/pdf
DOI  :  10.1088/1755-1315/235/1/012095

学科分类：社会科学、人文和艺术（综合）

来源: IOP

【 摘 要 】

Many schools do not organize the biological practice with the topic of bacterial inoculation and Gram staining, especially remote schools in the archipelago due to the difficulty of obtaining bacterial growth medium (Nutrient Agar/NA). This study aims to find alternative sources of nutrients used in NA medium that can be used by teachers in remote areas of the archipelago to be able to carry out bacterial and Gram staining breeding practicum. This research consisted of several stages, namely: (1) sample preparation; (2) manufacture of NA control medium; (3) the production of an alternative medium by the treatment of the use of Layang fish extract (Decapterus russelli) with peptone and without peptone; (4) bacterial inoculation with three different sources; and (5) the calculation of bacterial colonies. The data obtained were analyzed by using Descriptive Analysis. The results showed that the medium of "Narik Layang" using the extract of Layang fish (Decapterus russelli) could be used as an alternative to NA medium that using beef extract. Based on these results, the biology teacher can use the extract of Layang fish as a substitute for beef extract in the manufacture NA medium to carry out bacterial inoculation and Gram staining practicum.