Glucosamine production from palmyrah (Borassus flabellifer L.) seeds (a study of precursor type and concentration)

International Conference on Green Agro-industry and Bioeconomy

农业科学;工业技术（总论）;经济学

Rahmah, N.L.^1 ; Hidayat, N.^1 ; Hajar, B.K.^1

Department of Agro-industrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, Indonesia^1

关键词: Ammonium salt;    Extraction process;    Human bodies;    Least Significant Difference tests;    Nested design;    Precursor concentration;    Qualitative test;    Retention factors;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/230/1/012027/pdf
DOI  :  10.1088/1755-1315/230/1/012027

来源: IOP

【 摘 要 】

Glucosamine is an amino-sugar compound that is a precursor in synthesis repairment of cartilage and joints in human body. Glucosamine can be produced in the body as well as obtained from animal and plant sources. One of the plant sources that contain glucosamine is the seed of palmyrah. To obtain glucosamine from palmyrah seeds is required extraction process. The addition of ammonium salt precursor to the extraction stage could increase the production of glucosamine. The aim of this research was to determine the influence of the type and concentration of ammonium salt precursor to the glucosamine content from seeds of palmyrah fruit. This research used nested design with two factors, consisting of type and concentration of precursor. The main factor of precursor type (as first factor) consisting of three levels, namely NH4Cl, (NH4)2CO3, and (NH4)2SO4, although nested factor of precursor concentration (as second factor) consisting of two levels. The glucosamine content was tested by spectrophotometry analysis. The effect of treatment was analyzed using analysis of variance and was continued with the Least Significant Difference test. Glucosamine was tested qualitatively by Thin Layer Chromatography. The results showed that the best treatment of precursor types were found in the addition of NH4Cl 4.5 M; (NH4)2CO3 1.5 M; and (NH4)2SO44 M with glucosamine content1418.5; 1263.5 and 1078.5 ppm and yield 0.450; 0.380; and 0.627% respectively.The qualitative test using TLC showed all samples of extracts have similarities of retention factor with glucosamine standard.