Antibody Titers in The Sheep which were Immunated Antigen of Whole Protein from Third Instar Larvae Musca domestica

12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry

生物科学;化学

Ariantini, B.^1 ; Ratnani, H.^2 ; Luqman, E.M.^3 ; Hastutiek, P.^4

Veterinary Medicine Faculty, Airlangga University, Surabaya

60115, Indonesia^1

Department of Veterinary Reproduction, Veterinary Medicine Faculty, Airlangga University, Surabaya

60115, Indonesia^2

Department of Veterinary Embriology, Veterinary Medicine Faculty, Airlangga University, Surabaya

60115, Indonesia^3

Department of Veterinary Parasitology, Veterinary Medicine Faculty, Airlangga University, Surabaya

60115, Indonesia^4

关键词: Antibody titers;    Descriptive analysis;    ELISA test;    Enzyme linked immunosorbent assay;    Immune response;    Musca domestica;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012022/pdf
DOI  :  10.1088/1755-1315/217/1/012022

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

This research aimed to know antibody titers in the sheep which were injected antigen of whole protein from third instar larvae Musca domestica by Enzyme-Linked Immunosorbent Assay (ELISA), that giving the antigen could cause increasing antibody titers in the sheep. A male sheep about two years old were immunated with whole protein from larvae Musca domestica which was added Freund's Complete Adjuvant and done three times injection again (booster) with range of time once-two weeks and addition of Freund's Incomplete Adjuvant. Taking sheep blood before and after immunitation was done to get serum as composition of ELISA test. Data which were got in this research, tabulated with descriptive analysis. The result of this research showed that there was a little of increasing antibody titers. The most titers at booster 1, then gradually had drastic decreasing. Based on the result of ELISA test can be concluded that antigen of whole protein from larvae Musca domestica can't stimulate immune response in the sheep.