Protein Profiles of Escherichia coli Inactivation Results with Gamma Irradiation on Doses 600-800 Gy

9th Annual Basic Science International Conference 2019

自然科学（总论）

Handayani, T.^1 ; Rachim, A.^3 ; Priyoatmojo, D.^1 ; Tetriana, D.^2 ; Sugoro, I.^1

Center for Isotope and Radiation Application (PAIR)-BATAN, Jl. Lebak Bulus Raya No. 49, Lebak Bulus, Kota Jakarta Selatan

12440, Indonesia^1

Center for Metrology and Safety Radiation (PTKMR)-BATAN, Jl. Lebak Bulus Raya No. 49, Lebak Bulus, Kota Jakarta Selatan

12440, Indonesia^2

Department of Chemistry-FST UIN Syarif Hidayatullah, Jl. Ir. H. Djuanda No. 95, Ciputat, Tangerang Selatan

15412, Indonesia^3

关键词: and gamma;    Bacterial cells;    E.Coli inactivation;    Gamma irradiation;    Protein antigens;    Protein profiles;    Total protein;    Total protein contents;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/6/062009/pdf
DOI  :  10.1088/1757-899X/546/6/062009

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Escherichia coli is one of the main bacteria of the coliform type which can cause mastitis in dairy cows. Efforts to prevent mastitis are by the vaccination program. In this study, the E. coli (S1) bacterial cell strains tested were the result of isolation from milk from dairy cows infected with mastitis. Aims of the research were to know the protein profile of E. coli inactivation results with gamma irradiation on doses 600-800 Gy. Bacterial cells were inactivated by gamma irradiation at doses of 600, 650, 700, 750 and 800 Gy. The parameters analyzed are the viability of bacteria by TPC method, total protein by Lowry method and protein profile by electrophoresis using SDS-PAGE. The results showed that the dose to inactivate E. coli bacterial cells with gamma irradiation was 700 Gy. The total protein content of E. coli cells inactivated by gamma irradiation at doses of 0 Gy (control), 600 Gy, 650 Gy, 700 Gy, 750 Gy, 800 Gy respectively was 4.60 mg/ml; 4.77 mg/ml; 13.77 mg/ml; 9.43 mg/ml; 4.40 mg/ml; and 5.60 mg/ml. Protein bands of E. coli bacteria cells from inactivation with gamma irradiation were not different from before irradiation and protein antigens were still detected in 22kDa, 26kDa, 35kDa, and 58kDa.