Microfluidic Paper based Analytical Device (?pads) for Analysis of Benzoat Acid in Packaged Beverages

9th Annual Basic Science International Conference 2019

自然科学（总论）

Sari, M.I.^1^2 ; Andayani, Ulfa^1 ; Anneke^3 ; Andini^1^2 ; Sabarudin, Akhmad^1

Department of Chemistry, Faculty of Science, Brawijaya University, 65145, Indonesia^1

Academy of Food and Pharmacy Analyst of Putra Indonesia, Malang

65123, Indonesia^2

Faculty of Natural Resources, Prince of Songkla University, Hat Yai Songkla

90112, Thailand^3

关键词: Acid concentrations;    Analytical results;    Correlation coefficient;    Detection zones;    Hydrophilic channels;    Paper-based analytical devices;    Quantitative detection;    Spectro-photometric method;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/3/032028/pdf
DOI  :  10.1088/1757-899X/546/3/032028

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Microfluidic Paper-based Analytical Devices (μPADs) are a simple analytical platform that satisfy combination of low cost, portability, and ease-to-use with selectivity, sensitivity, and accuracy. In this experiment, the μPADs devices are prepared using a chromatographic paper and designed at appropriate pattern prior to printing by mean of a solid ink (wax) printer to produce hydrophobic barriers and hydrophilic channels. Then, the μPADs are heated at 120C for 3 min to allow a wax penetrates in the both side of a paper. The resulted μPADs are used for the detection of benzoic acid through alkalimetry principle. For this purpose, the devices are implanted with 5M NaOH in the detection zone and a 2% phenolphthalein indicator in the sample zone before dried in open air. When the devices have dried, the μPADs are ready to be used to detect benzoic acid. To avoid color interferences, the samples are distilled before introduced to the sample zone for quantitative detection. The result soft the color changes can be immediately seen by naked eye, and their intensities are quantified by the Image J software. Excellent linearity is achieved in the range of benzoic acid concentration of 20-120 ppm with the linear equation of Y = 0.0102 x + 0.0077 and the correlation coefficient (R2) of 0.9999. The optimized μPADs devices are successfully applied to the quantitative analysis of benzoic acid in the 5 kinds of commercially packaged beverages. There is no significantly different of the analytical results obtained by the PADs in comparison to the spectrophotometric method. Overall, the results obtained in this study indicate that the μPADs devices are a reliable tool for high throughout and on-site determination of benzoic acid content in commercial drinks.