Printed Low-Cost Microfluidic Paper-based Analytical Devices for Quantitative Detection of Vitamin C in Fruits

9th Annual Basic Science International Conference 2019

自然科学（总论）

Andini^1^2 ; Andayani, Ulfa^1 ; Anneke^3 ; Sari, Meiria Istiana^1^2 ; Sabarudin, Akhmad^1

Department of Chemistry, Faculty of Science, Brawijaya University, 65145, Indonesia^1

Academy of Food and Pharmacy Analyst of Putra Indonesia Malang, 65123, Indonesia^2

Faculty of Natural Resources, Prince of Songkla University, Songkhla Hat Yai

90110, Thailand^3

关键词: Chromatography paper;    Dichlorophenolindophenol;    Indonesia;    Intensity levels;    Low costs;    Paper-based analytical devices;    Quantitative detection;    Vitamin C;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/3/032002/pdf
DOI  :  10.1088/1757-899X/546/3/032002

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Microfluidic paper-based analytical device has been fabricated and subsequently applied to determine the Vitamin C level of the non-seasonal fruit i.e pineapples, oranges, guavas, and apples in Indonesia. PAD was prepared by creating a hydrophobic barrier on Whatman Chromatography paper No. 1 in a certain pattern printed using Xerox Colorcube 8580 DN-2. 0.05 l of 2,6-Dichlorophenolindophenol was dispersed into a detection area on the PAD devices prior to the addition of 0.2 l of fruits juice samples. The barrier allowed the fruits juice to flow and alter the color of the reagents through a redox reaction. Vitamin C in fruit juice altered the color of 2.6-Dichlorophenolindophenol reagent into a pinkish color. The changing of the reagent color was then processed using Image J software to determine the RGB intensity level. The results showed that the average amounts of Vitamin C in pineapples, oranges, guavas, and apples are 0.05%, 0.05%, 0.08%, and 0.04% respectively.