The Inhibitory effect of Natural materials Black Tea Theaflavins on MMPs-2 and MMPs-9 activities in Dentine

2018 6th International Conference on Mechanical Engineering, Materials Science and Civil Engineering

材料科学;机械制造;土木建筑工程

Li, Hong^1 ; Wang, Yina^1 ; Xu, Jiongyuan^1

Hangzhou Dental Hospital, School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, China^1

关键词: Concentration ranges;    Concentration-dependent manners;    Inhibitory effect;    Natural materials;    Natural products;    Negative control;    Protein extract;    Theaflavins;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/542/1/012086/pdf
DOI  :  10.1088/1757-899X/542/1/012086

来源: IOP

【 摘 要 】

The aim of the present study is to investigate the inhibitory effect of black tea theaflavins(TF) on gelatinases (MMP-2, MMP-9) in human dentine. Protein extracts from demineralized human dentine powder were treated with TFs concentration range from 0.1-1.6 mg/mL; Samples treated with EDTA was used as a positive control while untreated sample as a negative control; Zymography was utilized to assess MMP-2 and MMP-9 activities in dentin. The results demonstrated that untreated demineralized dentine showed the strongest band of MMPs activity on zymography, while EDTA treatment completely inhibited MMPs activity; MMPs activity was inhibited by TFs in a concentration dependent manner. We conclude that theaflavins inhibited MMP-2 and MMP-9 activities, suggesting that natural product theaflavins could be a potential supplementary therapy both for prevention and treatment of dentine caries.