Glutathione from Saccharomyces cerevisiae as By-Product of Second Generation Bioethanol from Oil Palm of Empty Fruit Bunch Fiber

International Conference on Science and Innovated Engineering

工业技术（总论）;自然科学（总论）

Sudiyani, Yanni^1 ; Faizal, F.A.^2 ; Muryanto^1 ; Firmansyah, I.^1 ; Setiawan, A.A.R.^1

Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Kawasan PUSPIPTEK Serepong, Tangerang Selatan

15314, Indonesia^1

Faculty of Mathematics and Science, Universitas Islam Negeri Syarif Hidayatulla, Tangerang Selatan

15314, Indonesia^2

关键词: Empty fruit bunch fibers;    Fermentation broths;    Fermentation waste;    Glutathione concentration;    Oil palm empty fruit bunch (OPEFB);    Phosphate buffers;    Second generation;    Second generation bioethanol;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/536/1/012142/pdf
DOI  :  10.1088/1757-899X/536/1/012142

来源: IOP

【 摘 要 】

Glutathione is an antioxidant agent can be applied in various fields such as pharmacy, cosmetic, foodstuff and another field. The objective of this research is to obtained glutathione from Saccharomyces cerevisiae in fermentation waste as by product of second generation of bioethanol (G2). After bioethanol process for 72h, centrifuge method was used to separate the yeast cells from residual fermentation broth of bioethanol. The yeast cells were extracted by maceration method using variation solvent with 25% ethanol solvent, 40% ethanol in water, H3PO4 0.1 M and Phosphate Buffer pH 7.2. Glutathione concentration was tested with alloxan method using Spectrophotometer UV-Vis and characterization using LC-MS. The result showed fermentation of oil palm empty fruit bunch (OPEFB)15% obtained ethanol 39 g/L and total cell after 72 h showed 2.15 x 108. The highest glutathione concentration had obtained from phosphate buffer pH 7.2 with extraction time 90 min which is equal 1449.52 mg/L.