The immune responds of balb/C Mice on antigen recombinant fim-C inclusion bodies salmonella typhi protein emulsified with alumina adjuvant

13th Joint Conference on Chemistry

Nurjayadi, Muktiningsih^1 ; Ariastuti, Dwi^1 ; Agustini, Kurnia^2 ; Sulfianti, Asri^2 ; Mangunwardoyo, Wibowo^3

Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim asj'Ari, Jl. Rawamangun Muka Jakarta Timur

13220, Indonesia^1

Laboratory for the Development of Industrial Technology for Agriculture and Biomedical (LABTIAP), BPPT, Indonesia^2

Department of Biology, Faculty of Mathematic and Sciences, Universitas Indonesia, Depok

16424, Indonesia^3

关键词: BALB/c mice;    Control groups;    Immune response;    Immunoblotting methods;    Immunogenicity;    Inclusion bodies;    Salmonella typhi;    Typhoid fever;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012089/pdf
DOI  :  10.1088/1757-899X/509/1/012089

来源: IOP

【 摘 要 】

Typhoid fever is still a world health problem, especially in developing countries, including Indonesia. One way to prevent typhoid fever is by vaccination. Different variations have been developed to improve the efficacy of the process. An important part of the current focus is the appropriate adjuvant selection in enhancing the immune response. The purpose of this study is to evaluate the ability of Fim-C Salmonella typhi protein emulsified with adjuvant alumina as candidate vaccine typhus against the immune response generated of Balb-C mice. This study was conducted on 25 male Balb-C mice grouped into 5 groups. That was a normal group (not treated); Control group 1 (given PBS 1x); control group 2 (given alumina); sample group-1 (given recombinant protein Fim-C inclusion bodies S. Typhi and PBS 1x); sample group-2 (given recombinant protein Fim-C inclusion bodies S. Typhi and alumina). Anti-Fim-C antibodies produced by Balb-C mice group 1 (KS-1) and group 2 (KS-2) samples began to increase after the test animals were given Fim-C antigen by 20 μg and experienced a significant increase after given an antigen of 40 μg. The results of immunogenicity testing by ELISA and western immunoblotting methods showed that the anti-Fim-C antibody produced by Balb-C mice was precise to recombinant Fim-C inclusion bodies S. Typhi protein as antigen. Based on the data, it was concluded that the Fim-C Inclusion bodies S. Typhi protein emulsified with Alumina adjuvant can produce a good and specific immune response, potentially as a candidate of typhoid vaccine.