Research on some factors affecting extraction of chlorophyll from mulberry leaves (Morus alba)

3rd International Conference on New Material and Chemical Industry

材料科学;化学工业

Tran, Q.H.^1 ; Pham, T.Q.^1 ; Vu, H.T.^2 ; Le, D.X.^3 ; Tran, O.T.^4 ; Ngo, A.Q.^1 ; Nguyen, T.D.^5 ; Hoang, B.T.^3 ; Do, S.T.^1

Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District Hanoi, Viet Nam^1

Faculty of Hydro-Meteorology, HCMC University of Natural Resources and Environment, Le Van Sy Street, Tan Binh District, Ho Chi Minh City, Viet Nam^2

Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District Hanoi, Viet Nam^3

Vietnam National University of Agriculture, Gia Lam District Hanoi, Viet Nam^4

NTT Institute of High Technology, Nguyen Tat Thanh University, 300A Nguyen Tat Thanh, District 4, Ho Chi Minh City, Viet Nam^5

关键词: Central composite designs;    Dependent variables;    Extraction time;    Independent variables;    Optimal conditions;    Optimal results;    Second-order polynomial equations;    Total chlorophyll;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/479/1/012004/pdf
DOI  :  10.1088/1757-899X/479/1/012004

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

The purpose of this report is to investigate some factors affecting extraction of chlorophyll from mulberry leaves (Morus alba). In the three (3) types of solvents used (acetone, ethanol, and methanol), 90% acetone was the appropriate solvent to extract chlorophyll from mulberry leaves giving a total chlorophyll concentration of 0.341%, in which chlorophyll achieved a concentration of 63%. Some factors such as temperature, material/solvent ratio, extraction time were also investigated. This survey was studied for optimum use a Box-Wilson central composite design consisting of 03 independent variables (temperature, materials/solvent ratio and extraction time) and two dependent variables (total chlorophyll and chlorophyll a ratio. Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Select the optimal conditions are T=26.75 °C, material/solvent ratio 12.67 %, τ = 24.3 h. Optimal results are found out conditions to obtain the highest chlorophyll total (Y1(opt) = 0.354%) while chlorophyll a ratio at the desired concentration Y2 > 60%.