Improvement of cell freezing technologies: towards a fully closed process

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

生物科学;医药卫生

Aurore Polès-Lahille ; Brigitte Lafuente ; Virginie Perrier ; David Balbuena ; Didier Peyret

Others  :  http://www.biomedcentral.com/content/pdf/1753-6561-5-S8-P1.pdf
PID  :  17453

来源: CEUR

【 摘 要 】

Cell culture from vials usually includes an open phase performed under laminar flow hoods. Even if disposable and small closed systems are available, at least the first cell culture step after vial thawing, which is a centrifugation, is performed in disposable but open containers. This step is critical as the risk of contamination is high and it also has a direct impact on process timelines. [First Paragraph]