Utilization of multifrequency permittivity measurements in addition to biomass monitoring

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

生物科学;医药卫生

Christoph Heinrich ; Tim Beckmann ; Heino Büntemeyer ; Thomas Noll

Others  :  http://www.biomedcentral.com/content/pdf/1753-6561-5-S8-O10.pdf
PID  :  26630

来源: CEUR

【 摘 要 】

In recent years measurement of permittivity signal has been increasingly used for online biomass monitoring of cell cultures. Breweries use it as an established method for fermenter inoculation and bioprocess control for instance [1]. In the case of animal cell cultures the correlation between permittivity and viable cell densities determined offline varies along cultivation time. Hence, several authors have used the permittivity signal as an indirect method for measuring oxygen and glutamine consumption as well as intracellular nucleotide phosphate concentrations [2,3]. The latest generation of biomass monitoring devices allows parallel measurement of permittivity at a range of frequencies leading to an improvement in the correlation between biomass and permittivity and providing a tool to further explore other aspects related to the physiological state of the cells.[First Paragraph]